{"id":1120,"date":"2015-02-11T07:58:30","date_gmt":"2015-02-11T07:58:30","guid":{"rendered":"http:\/\/www.ernestohernandez.org\/?page_id=1120"},"modified":"2015-02-11T07:59:03","modified_gmt":"2015-02-11T07:59:03","slug":"molecular-identification-of-microbes-in-treated-sludge","status":"publish","type":"page","link":"http:\/\/www.ernestohernandez.org\/?page_id=1120","title":{"rendered":"Molecular identification of microbes in treated sludge"},"content":{"rendered":"<p style=\"text-align: justify;\">This CONACYT&#8217;s sponsored project aimed at isolating and identifying fermentative microbes that survived after being boiled and were able to feed on phenol. Traditional microscopy techniques were complemented with two 16S rRNA molecular-based methods: clone library and TGGE fingerprinting.<\/p>\n<div id=\"attachment_405\" style=\"width: 959px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-405\" data-attachment-id=\"405\" data-permalink=\"http:\/\/www.ernestohernandez.org\/?attachment_id=405\" data-orig-file=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?fit=949%2C370\" data-orig-size=\"949,370\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;}\" data-image-title=\"Molecular identification\" data-image-description=\"&lt;p&gt;Molecular identification by applying clone library and TGGE fingerprinting&lt;\/p&gt;\n\" data-image-caption=\"&lt;p&gt;Molecular identification via clone library and TGGE fingerprinting.&lt;\/p&gt;\n\" data-medium-file=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?fit=300%2C116\" data-large-file=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?fit=640%2C250\" class=\"wp-image-405 size-full\" src=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?resize=640%2C250\" alt=\"Molecular identification via clone library and TGGE fingerprinting.\" width=\"640\" height=\"250\" srcset=\"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?w=949 949w, https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?resize=300%2C116 300w, https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2013\/10\/Molecular-identification.png?resize=500%2C194 500w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><p id=\"caption-attachment-405\" class=\"wp-caption-text\">Molecular identification via clone library and TGGE fingerprinting.<\/p><\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>This CONACYT&#8217;s sponsored project aimed at isolating and identifying fermentative microbes that survived after being boiled and were able to feed on phenol. Traditional microscopy techniques were complemented with two 16S rRNA molecular-based methods: clone library and TGGE fingerprinting. &nbsp;<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-1120","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/P6rw5S-i4","jetpack-related-posts":[{"id":335,"url":"http:\/\/www.ernestohernandez.org\/?page_id=335","url_meta":{"origin":1120,"position":0},"title":"Research","author":"Ernesto Hernandez","date":"August 15, 2013","format":false,"excerpt":"\"Nature is my\u00a0inspiration, it\u00a0motivates me to apply the tenets of chemical engineering to solve some of the current challenges faced by humankind \u2013I may call this, bioinspired chemical engineering\" \u00a0 Value-added chemicals from biomass Functional food from biomass bioprocessing Microbial enhanced oil recovery Bioenergy from cheese industry Molecular identification of\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1115,"url":"http:\/\/www.ernestohernandez.org\/?page_id=1115","url_meta":{"origin":1120,"position":1},"title":"Microbial enhanced oil recovery","author":"Ernesto Hernandez","date":"February 11, 2015","format":false,"excerpt":"This was a technology transfer UK industry-government sponsored project, aiming at developing and implementing a new technology to monitor water-injection chemistries to increase oil recovery through down-borehole bacterial activity. This technology would facilitate the flow of oil in deep underground reservoirs at high pressures -extreme environments. We studied the anaerobic\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":351,"url":"http:\/\/www.ernestohernandez.org\/?page_id=351","url_meta":{"origin":1120,"position":2},"title":"Published gene sequences","author":"Ernesto Hernandez","date":"August 15, 2013","format":false,"excerpt":"Hernandez, J.E., Blanco, D. and Edyvean, R.G. (2007). EF373707 to EF590285. Uncultured bacterium clone GM5F-RD2 16S ribosomal RNA gene, partial sequence. gi|124494219|gb|EF373707.1|. Hernandez, J.E., Blanco, D. and Edyvean, R.G. (2007). EF437217. Uncultured bacterium isolate TGGE gel band GM5F-RD2 16S ribosomal RNA gene, partial sequence. gi|133753037|gb|EF437217.1|. Bachmann, R. T., Eboigbodin, K.\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":359,"url":"http:\/\/www.ernestohernandez.org\/?page_id=359","url_meta":{"origin":1120,"position":3},"title":"Presentations and posters at conferences","author":"Ernesto Hernandez","date":"August 15, 2013","format":false,"excerpt":"Blanco, D., Hernandez, J.E., Mulas, D. and Gil, M.V. (2007). Genotypic characterization of phenol degrading microbes by applying Temperature Gradient Gel Electrophoresis (TGGE). XXXVI Congress of the Spanish Genetics Society, Leon, Spain. Hernandez, J. E & Edyvean, R. G. (2005) Inhibition of biogas production by substituted phenolic compounds in enriched\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]},{"id":1658,"url":"http:\/\/www.ernestohernandez.org\/?page_id=1658","url_meta":{"origin":1120,"position":4},"title":"Error autoexterminador","author":"Ernesto Hernandez","date":"November 29, 2016","format":false,"excerpt":"https:\/\/youtu.be\/SqhkyJslSBg Informaci\u00f3n importante SI QUIERES APOYAR: MANITA ARRIBA Y COMPARTELO, GRACIAS!!! \u2665Suscribirse al canal Caos \u2665Facebook: https:\/\/www.facebook.com\/iCaosv\/v\/ \u2665Twitter:https:\/\/twitter.com\/iCaosv \u2665Contactar: vchaosnow@gmail.com \u2665Referencias, notas, etc: http:\/\/www.ernestohernandez.org\/?page_id=1658 Enfermedades raras o enfermedades geneticas? aqu\u00ed tienes una donde un error activar\u00eda la automutilaci\u00f3n o autodestrucci\u00f3n. Buscabas enfermedades hereditarias ejemplos? Pues aqu\u00ed hay uno Por favor\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"Caos Channel Brand Logo Ernesto Hernandez","src":"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2016\/11\/Caos-channel-art.jpg?fit=1200%2C675&resize=350%2C200","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2016\/11\/Caos-channel-art.jpg?fit=1200%2C675&resize=350%2C200 1x, https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2016\/11\/Caos-channel-art.jpg?fit=1200%2C675&resize=525%2C300 1.5x, https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2016\/11\/Caos-channel-art.jpg?fit=1200%2C675&resize=700%2C400 2x, https:\/\/i0.wp.com\/www.ernestohernandez.org\/wp-content\/uploads\/2016\/11\/Caos-channel-art.jpg?fit=1200%2C675&resize=1050%2C600 3x"},"classes":[]},{"id":5,"url":"http:\/\/www.ernestohernandez.org\/?page_id=5","url_meta":{"origin":1120,"position":5},"title":"About","author":"Ernesto Hernandez","date":"December 13, 2011","format":false,"excerpt":"Ernesto Hernandez is a life scientist and chemical engineer working at Canterbury Christ Church University. He started out his career as a distinguished technician working in the field of electromechanics. In his spare time, he indulged his passion for studying biology. This long-standing endeavour led him to win the first\u2026","rel":"","context":"Similar post","block_context":{"text":"Similar post","link":""},"img":{"alt_text":"","src":"","width":0,"height":0},"classes":[]}],"_links":{"self":[{"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/pages\/1120","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1120"}],"version-history":[{"count":1,"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/pages\/1120\/revisions"}],"predecessor-version":[{"id":1121,"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=\/wp\/v2\/pages\/1120\/revisions\/1121"}],"wp:attachment":[{"href":"http:\/\/www.ernestohernandez.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1120"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}